New Super-Resolution, Light-Sheet, and FCS-Methods at Scilifelab

The Advanced Light Microscopy Facility, ALM, at Scilifelab gives a two-week PhD-level course on four new imaging and fluorescence spectroscopy techniques:

Depletion-based Super-Resolution Imaging

• STED-imaging, 2D and 3D, 30-50 nm resolution
• In Living Cells – MoNaLISA, 1-2 Hz, 100 x 100 mm field of view

MINFLUX

• Imaging with 3 nm resolution
• Tracking single molecules at 10 kHz

Light-Sheet Imaging

• Long time-lapse imaging of live model organisms with low phototoxicity
• Ultrafast volumetric imaging of cells with lattice light-sheet microscopy

FCS-Methods

• Measurements of concentrations, diffusion coefficients, molecular dynamics and interactions, in solution or living cells
• Cross-correlation, FRET-, STED-, and line-scan-FCS are covered

Lecturers:

Steven Edwards

Erdinc Sezgin

Ana Agostinho

Francesca Pennacchietti

Hans Blom

Stefan Wennmalm

Dates: Monday October 14^th – Friday October 25^th 2024

Credits: 3 hp for PhD students

Location: Gamma 3, Scilifelab, Solna

Registration: stewen@kth.se latest September 30^th